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KMID : 0620919970290040235
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Experimental & Molecular Medicine
1997 Volume.29 No. 4 p.235 ~ p.237
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A simple protocol of DNA sequencing with 10% formamide for dissolving G/C compression
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Yeon Woong Kim/Kee Ryeon Kang
Yeon Woong Kim
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Abstract
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Formamide has been widely used in urea/polyacrylamide gel to solve the compression problems that are occasionally found during the DNA sequencing of G/C rich regions. In this study, however, 10% formamide was added in annealing solution in stead of adding to the gel. The compressions were unfolded efficiently with a more rapid annealing reaction on ice in the presence of 10% formamide.
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KEYWORD
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formamide, DNA sequencing, T7 polymerase,
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